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DSMZ

DSMZ 874 . FERROPLASMA ACIDIPHILUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Acidiplasma aeolicum DSM 18409

* Modification: Replace trace elements with 1.00 ml/l of trace element solution SL-10 (see medium 320). Supplement medium prior to inoculation with 1.50 g/l potassium tetrathionate added from a stock solution sterilized by filtration.

Growth conditions: 40 °C

Main sol. 874 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 930 ml - -
Solution B 50 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
1 Add solutions B and C to solution A, adjust pH of the combined solution to 1.6 - 1.8 with H2SO4, then sterilize using membrane filter and dispense in sterile culture vessels. Prior to use, add the appropriate volume of solution D sterilized by autoclaving.

Solution A #

930
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.4 g 0.43 1.745
(NH4)2SO4 0.2 g 0.215 1.627
KCl 0.1 g 0.108 1.442
K2HPO4 0.1 g 0.108 0.617
Potassium tetrathionate* 1.5 g - -
Distilled water 930.0 ml - -

Solution B #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 25 g 500 1798.46
H2SO4
(1 N) 		40 ml - -
Distilled water 10 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution* 10 ml - -
Trace element solution SL-10* 1 ml - -

Solution D #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(OXOID) 		0.2 g 20 -
Distilled water 10.0 ml - -

Trace element solution #

1000
Solution occurs in 1 other media (first defined in Medium 158).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O 1.93 g 1.93 7.14
MnCl2 x 4 H2O 0.18 g 0.18 0.91
Na2B4O7 x 10 H2O 0.45 g 0.45 -
ZnSO4 x 7 H2O 22.00 mg 0.022 0.077
CuCl2 x 2 H2O 5.00 mg 0.005 0.029
Na2MoO4 x 2 H2O 3.00 mg 0.003 0.012
VOSO4 x 5 H2O 3.80 mg 0.004 0.015
CoSO4 x 7 H2O 2.00 mg 0.002 0.013
Distilled water 1000.00 ml - -

Trace element solution SL-10 * #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 07.04.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 1.6 - 1.8
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 4
Strains with modifications: 2
Acidiplasma aeolicum DSM 18409

  1.5 g Potassium tetrathionate
-  Trace element solution
+  1 ml Trace element solution SL-10

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 25 89.923
Potassium tetrathionate 1.5 4.959
MgSO4 x 7 H2O 0.4 1.623
(NH4)2SO4 0.2 1.514
Yeast extract 0.2 -
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
temperature mesophilic 2 strains
temperature thermophilic 2 strains